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Serial Dilutions. A dilution series is a succession of step dilutions, each with the same dilution factor, where the diluted material of the previous step is used to make the subsequent dilution. This is how standard curves for ELISA can be made. To make a dilution series, use the following formulas: Move Volume = Final Volume / (DF -1). Serial Dilution Method: Definition and Procedure Explained http://www.biologyexams4u.com/2013/12/serial-dilution-protocol-pdf.html.